Santiago de Chile

2014 Regenerative Medicine

kristen-comella

Dr Kristen Comella

Regenerative Medicine for Degenerative Diseases

The field of regenerative medicine is rapidly growing with an emphasis on stem cell therapies and the promise of cures for everything from acute injuries to chronic degenerative diseases.  The list of indications is expanding as more studies are published to demonstrate safety and efficacy.  Regenerative medicine is the process of replacing or regenerating human cells, tissues or organs to restore or establish normal function.  The concept is that damaged tissue can be restored by using the body’s own healing mechanism to promote repair.  This new branch of medicine may change the course of chronic diseases and standard clinical therapies.  However, with any new therapies, this must be approached cautiously and with scientific data to support the treatment.  This session will discuss the use of stem cell therapies in degenerative diseases and the challenges of bringing these products to market.

david-harrell

Dr David Harrell Cellular

Composition of Bone Marrow with a Focus on Stem and Progenitor Cells!

Activities of Bone Marrow Stem and Progenitor Cells! Importance of Cellular Mobilization! Course of Traumatized Tissue! Importance of Neovascularization! Cells Crucial for Tissue Regeneration! Importance of Paracrine Effect and the Microenvironment! Relate Basic Science to Clinical Practice

joseph-purita

Dr Joseph Purita

Tittle Latest developments in PRP and Stem Cells injections for orthopedic applications.

The presentation concerns the use of PRP and Stem cell injections for treatment of musculoskeletal conditions.  We wish to take the discussion to the next level by discussing the cutting edge of the cutting edge.  Our clinic has made extensive use of Platelet Rich Plasma in conjunction with Bone Marrow Stem Cells (BMAC) and Adipose Stem Cells (SVF) and fat grafts.  We have now expanded our practice to use stem cells which until recently were thought to exist in therapy only.  All of these components are harvested from the patient, minimally manipulated, and utilized the same day essentially keeping within FDA guidelines.

todd-malan

Dr Todd Malan

The Practical Application of Adipose Derived Stromal Vascular Fraction (SVF) in Cosmetic and Reconstructive Surgery.

In this presentation I will discuss these emerging technologies and techniques and describe our experience, as the first adipose derived stem cell treatment center in the U.S., in obtaining high yield lipoaspirate tissue sample utilizing the Body-Jet™ (HumanMed, Schwerin, Germany) WAL device as compared to traditional liposuction, syringe based liposuction, and competing technologies. In addition I will discuss our experience utilizing automated SVF processing devices such as the Stem Source™ device by Cytori Inc. (San Diego, CA, USA.) and LipoKit (MediKhan, Seol, Korea) as compared to standardized manual processing methods for the purpose of obtaining graft specimen enhanced with SVF. Flow Cytometry, Growth Kinetics Assay, and Image Analysis data will be presented comparing SVF yield and MSC characterizations as seen when utilizing various techniques for harvesting and processing. A cost effective and clinically feasible solution for the routine storage of SVF enriched adipose tissue for future cosmetic as well as tissue engineering and regenerative applications will also be discussed in detail.

maritza-novas

Maritza Novas

Cutting-edge concepts to the practice of regenerative medicine. Understanding aging as a platform for tissue regeneration.

The prevailing concept in anti-aging medicine holds that the rate of aging of an individual depends on the wear vs regeneration ratio in any given tissue. The stem cell niches in the body are not exempt from this relationship. Stem cells age and their regenerative and modulating capacity decreases.En this dissertation we will expose in detail the biology of cellular aging and an overall view of manipulations that favor the equilibrium toward the conservation and regeneration of stem cell niches to prevent or delay aging.

2015 Regenerative Medicine

fernando-espina

Dr. Fernando Espina Otero

KEYNOTE SPEECH AND OPENING REMARKS

enrique-testart

Dr. Enrique Testart

USE OF MSC TO ENGINEER DEVELOPMENTAL PROCESSES FOR REGENERATIVE MEDICINE

Following the exemplifying context of cartilage and bone regeneration, this lecture will describe and discuss alternative approaches to evolve classical tissue engineering paradigms towards possibly more effective grafts, with the potential for a broader clinical use. The lecture will then propose and discuss the concept of engineering regenerative strategies by recapitulating developmental processes, exploiting the own body as the in vivo bioreactor. Rather than engineering a tissue, the strategy targets the use of cells (e.g., MSC) to engineer the different stages of a process which recapitulate events of development (e.g., endochondral ossification). The product will be a tissue containing all necessary and sufficient cues to remodel into the target repair tissue upon grafting. The perspective will also address issues related to scalability, process control and regulatory compliance in manufacturing cell-based products and highlight the need not only to automate, but also to streamline and simplify typical production processes

joseph-purita

Dr. Joseph Purita

BONE NATURE AND BLOOD NURTURE: SAME STROMA?

Contrasting the relatively well known hierarchy and roles of haematopoietic cells in the bone marrow, lineage and functional relationships among non-hematopoietic cells have remained elusive, despite the large interest raised by their haematopoietic and immune regulatory properties. Among other cell types, endothelial cells, neuroglial cells, mesenchymal progenitors and osteochondral cells have been proposed as key elements of the haematopoietic stem cell (HSC) niche in the bone marrow. However, the developmental and functional relationships of these cells have remained poorly characterised. Technological developments have allowed start dissecting them but have also evidenced limitations that need to be overcome in order to advance the field. We previously showed that bone marrow nestin+ cells innervated by sympathetic nerve fibers regulate normal haematopoietic stem cells. Our recent data has demonstrated that this circuitry is critically damaged in myeloproliferative neoplasms, diseases that were previously thought autonomously driven by mutated haematopoietic stem cells. We will discuss origins and functions of bone marrow mesenchymal stem cells (MSCs) and the potential therapeutic implications.

stephan-jarpa

Stephan Jarpa

MSC BASED THERAPY FOR SEVERE OSTEOARTHRITIS OF THE KNEE: THE ADIPOSE EXPERIENCE

Identify specific signature associated with MSC immunosupressive effects, Potency assay associated with osteoarticular applications: where do we stand, MSC for osteoarthritis. implication of IL1RA expression

carlos-guerrero-silva

Dr. Carlos Guerrero Silva

MESENCHYMAL STEM CELL TRANSPLANTATION TO TREAT MULTIPLE SCLEROSIS

Mesenchymal stem cells (MSCs) have potent immunomodulatory, tissue-protective, and repair-promoting properties in vitro and in animal models. Clinical trials support the safety and efficacy of MSC transplantation in several human conditions. Published experience in multiple sclerosis (MS) is modest. We recently completed a phase 1 study of autologous MSC transplantation in MS. 1-2×106 culture-expanded, bone-marrow-derived MSCs/kg were administered IV to 16 women and 8 men, 10 relapsing-remitting and 14 secondary progressive, mean age 46.5 and EDSS 5.2, and 25% with Gd-enhancing brain lesions. Mean cell dosage (requiring 1-3 passages) was 1.9×106 MSCs/ kg (range 1.3-2.0) with post-thaw viability >95%. Cell infusion was well tolerated. There were no treatment-related severe or serious adverse events, or indication of disease activation. Trend analysis using splines suggested benefit in a number of exploratory clinical, imaging, and laboratory assessments, which will help guide planned phase 2 testing

alejandro-guiloff

Dr. Alejandro Guiloff

STEM CELLS IMMUNOLOGY AND PATHOLOGICAL AND THERAPEUTIC IMPLICATIONS

Mesenchymal stem cells (MSCs) are specifically attracted to damaged tissue sites and are believed to closely interact with inflammatory factors and cells during tissue repair and regeneration., MSCs play a key role in regulating immune responses., The immune regulatory capacity of MSCs is plastic and varies according to the type and intensity of inflammation. Exogenously administered MSCs are believed to promoted tissue regeneration mainly through inflammation-induced cell empowerment

rodrigo-arencibia

Rodrigo Arancibia PhD

BRINGING MESENCHYMAL STEM CELLS INTO THE CLINIC

Mesenchymal Stromal Cells (MSCs) have immunemodulatory properites.MSCs have been infused intravenously, no acute infusional toxicity has been reported. Upon contact with blood, MSCs initiate activation of the complement and coagulation cascades.

2016 Regenerative Medicine

eduardo-mazzaro

Eduardo Mazzaro

Cutting Edge Concepts in the Practice of Regenerative Medicine

Understanding aging as a platform for tissue regeneration. The prevailing belief in anti-aging medicine is that the rate of aging in an individual depends on the wear vs. regeneration ratio in any given tissue. Not even the body’s stem cell niches are exempt from this relationship, as stem cells age and their regenerative and modulating capacity decrease. In her dissertation, Maritza Novas will expose in detail the biology of cellular aging, and provide an overall view of stem cell manipulations that favor conservation and regeneration of stem cell niches to prevent or delay the affects of aging.

karina-ravera

Dra. Karina Ravera

The Practical Application of Adipose Derived Stromal Vascular Fraction (SVF) in Cosmetic and Reconstructive Surgery.

BENITO NOVAS

HOW TO MARKET STEM CELLS ON SOCIAL NETWORK

Mr. Novas is an entrepreneur, marketer and visionary with a proven track record in healthcare development, marketing, promotion and management, as well as demonstrated leadership skills in operations management, process improvement, and change management and quality implementation

agusti-molins

Dr. AGUSTI MOLINS

IMMUNOLOGICAL ASSAYS AS POTENCY RELEASE CRITERIA FOR MESENCHYMAL STROMAL CELLS (MSCS)

Defining the main features of human MSC and immune effector cells for testing the immune regulatory potential • Defining the mechanisms of action that better characterize human MSC immunological properties • Defining robust markers and assays predictive of immunological potency • Defining the best pre-clinical research in animal models of adoptive cell therapy that provide important insights on mechanisms of action

joseph-purita

Dr. Joseph Purita

In this presentation, Dr. Purita will discuss concerns over the use of PRP and stem cell injections for treatment of musculoskeletal conditions. Dr. Purita will expand on the discussion by detailing the cutting-edge treatments he now offers to his clinic patients, including extensive use of platelet-rich plasma in conjunction with bone marrow stem cells (BMAC), adipose stem cells (SVF) and fat grafts. Dr. Purita has broadened his clinical practice to include stem cells, until very recently thought to exist only in therapy. All components of these treatments are harvested from the patient, minimally manipulated and used the same day in keeping with FDA guidelines.

david-audley

Mr. David Audley

Current Regulatory Environment for Regenerative Medicine

Learn about the current regulatory environment and how to safely, legally and ethically offer patients adult stem cell treatments in your practice.

  • What are the current adult stem cell (ASC) ethics and policies?
  • What are the current regulations on advanced stem cell therapies?
  • What is the FDA’s involvement?
  • What are the current stem cell clinical trials?
duncan-ross

Duncan Ross PhD

Exosomes: emerging roles in communication between blood cells and vascular tissues during atherosclerosis

Microvesicles, in general, and exosomes together with their delivered content in particular, are now being widely recognized as key players in atherosclerosis. We have previously reviewed the role of microvesicles in atherosclerosis pathogenesis, diagnosis and therapy. Here, we focus on the roles of exosomes and discuss their emergent role in mediating activation and response to inflammation, vessel infiltration and induction of coagulation. We will finally give an outlook to discuss novel detection techniques and systems biology based data analyses to investigate exosome-mediated cell-to-cell communication.

Dr. Enrique Testart

USE OF MSC TO ENGINEER DEVELOPMENTAL PROCESSES FOR REGENERATIVE MEDICINE

Following the exemplifying context of cartilage and bone regeneration, this lecture will describe and discuss alternative approaches to evolve classical tissue engineering paradigms towards possibly more effective grafts, with the potential for a broader clinical use. The lecture will then propose and discuss the concept of engineering regenerative strategies by recapitulating developmental processes, exploiting the own body as the in vivo bioreactor. Rather than engineering a tissue, the strategy targets the use of cells (e.g., MSC) to engineer the different stages of a process which recapitulate events of development (e.g., endochondral ossification). The product will be a tissue containing all necessary and sufficient cues to remodel into the target repair tissue upon grafting. The perspective will also address issues related to scalability, process control and regulatory compliance in manufacturing cell-based products and highlight the need not only to automate, but also to streamline and simplify typical production processes.

andrea-miranda

Dra. Andrea Miranda

A Review of New Facial Filler Techniques for Facial Rejuvenation

Over the recent years, the use of facial fillers for rejuvenation of the aging face has continued to increase in popularity. As the demand for noninvasive cosmetic enhancements increases, so does the need for new cosmetic techniques that will increase patient satisfaction while decreasing complications. In the past, facial fillers were mostly used for lip augmentation, nasolabial folds, and marionette lines. However, because of advances in cosmetic techniques and types of dermal fillers, cosmetic surgeons today are able to correct lines, wrinkles, and volume loss seen in crow’s feet, around the eyes, chin, and cheeks.

Global Stem Cells Group Symposium faculty members include physicians, practitioners, educators, researchers and product developers who engage in stem cell and regenerative medicine activities across a wide range of disciplines.